Sulfasalazine

SulfasalazineSulfasalazine is used to investigate bacterial folate metabolism and azo bond reduction, and to explore sulfonamide resistance and metabolic inhibition in microbial systems. It can be incorporated into culture conditions to evaluate susceptibility patterns or to study enzymatic cleavage of azo linked compounds by bacterial reductases. Sulfasalazine is a conjugate molecule composed of sulfapyridine linked to 5 aminosalicylic acid through an azo bond,

In high-performance liquid chromatographic separation OPA is used to derivatize amino acids and cytometric measurements of protein thiol groups

Mechanism of Action & Biological Function

Specifically

This induces paralytic muscle contraction and death

Brommonschenkel

How It Works: TCEP prevents thiol oxidation and does not interfere with maleimide chemistry

How It Works: Sodium taurodeoxycholate forms mixed micelles with phospholipids and cholesterol

Ideal for SDS-PAGE

No dilutions necessary

(S)-(+)-6-Methoxy-alpha-methyl-2-naphthaleneacetic acid

• Recovery of single-stranded biotinylated DNA for dideoxy sequencing

2 M H₂SO₄) in the correct volume — typical stop volumes ~ 1/10 to 1/4 the reaction volume — then read at 450 nm