PROTEINDEX™ Anti-DYKDDDDK Agarose 4 Fast Flow

PROTEINDEX™ Anti-DYKDDDDK Agarose 4 Fast FlowPROTEINDEX Anti DYKDDDDK Agarose 4 Fast Flow is an affinity chromatography medium for the purification of proteins that contain the peptide sequence DYKDDDDK (N Asp Tyr Lys Asp Asp Asp Asp Lys C). The ligand covalently immobilized on the matrix support is a recombinant mouse monoclonal IgG1 species that binds the same epitope as Sigma's Anti FLAG M2 Antibody, and it is highly specific for the peptide DYKDDDDK in a calcium independent manner. Commonly

Acetic Acid

and also accommodates tubing having 5/32" (4mm) or 3/32 inch (2

Anti-strip power of the Ni-Penta™ enables repetitive uses of the column without the need to recharge nickel ion

> 40 mg human IgG / mL of settled resins

8 mm Material (column)

5 of 12 mL sets

4 mm Column height 134

6 mm Column height 83 mm Material (column)

and 20 mM imidazole solution before elution with 300 mM imidazole

Concentration

PROTEINDEX™ rProtein G Agarose 4FF enables single-step purification of monoclonal and polyclonal antibodies with a greater than 95% purity

human and other mammalian samples